污渍
污渍
免疫印迹
规范化(社会学)
考马斯亮蓝
化学
蛋白质表达
家务
分子生物学
色谱法
染色
生物化学
生物
基因
社会学
遗传学
人类学
作者
Jennifer E. Gilda,Aldrin V. Gomes
出处
期刊:Methods in molecular biology
日期:2015-01-01
卷期号:: 381-391
被引量:80
标识
DOI:10.1007/978-1-4939-2550-6_27
摘要
Western blotting is a commonly used laboratory technique for semi-quantifying protein amounts. It is important when quantifying protein expression to account for differences in the amount of total protein loaded onto the gel using a loading control. Common loading controls include housekeeping proteins, such as β-actin or GAPDH, quantified by Western blot, or total protein, quantified using a stain such as Coomassie Brilliant Blue or Ponceau S. A more recently developed method for total protein quantification utilizes stain-free technology, which has a linear dynamic detection range and allows for protein detection on both gels and membranes. Here, we describe the theory and use of stain-free gels for total protein quantification and normalization of Western blots.
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