Determination and Purification of Polysaccharides in Fruit of Quince

Polysaccharides from fruit of quince was obtained by hotwater extraction and ethanol precipitation. The content of polysaccharide was determined by spectrophotometry.The content of polysaccharide assayed by phenol-vitriolic colorimetry at 490nm,was 67.28% with3.08% of RSD (n=4). Recovery value for the polysaccharides assayed was 101.2%. The concentrated polysaccharides was purified by polyamide column and the results showed that decolorization was significant.