The study on the expression of Fas and Bcl-2 with myocyte apoptosis during the cardiac injury induced by β-adrenergic over-activation

AIM: To investigate the expression of Fas and Bcl-2 and the significance of myocyte apoptosis during the cardiac injury induced by β-adrenergic over-activated in rats. METHODS: The rats were treated with subcutaneous injection of isoproterenol (ISO). Fas and Bcl-2 protein was identified by immunohistochemical technique. Apoptotic myocytes were stained in situ by TUNEL, Myocardial damage stained with hematoxylin and eosin was examined. RESULTS: The expression of Fas and Bcl-2 was both up-regulated (P0.01 and P0.01, and the degree in Fas expression was more higher (P0.01). At 6 and 72 hour with cardiac injury, the apoptosis index was 7.6±1.5、14.8±4.1 respectively. CONCLUSION: Our study suggests that the cardiomyocyte apoptosis is one of the important pathogenesis involved in cardiac insufficiency during the myocardium damage induced by the β-adrenergic over-activated, and there is significantly difference between the expression of Fas and Bcl-2 with the high level in the regulation of cardiomyocytes apoptosis.