The polysaccharides from Lentinus edodes,Plurotus sajorcaju,Flammulina velutiper,Pleurotus geesteranus,Agrocybe chaxingu and Ganoderma lucidum mycelium were extracted by boiling water method,and the content of polysaccharides was measured by Phenol-Sulfuric Acid Method.The results showed that the contents of polysaccharides in the six mushroom mycelium were 4.84 %,3.45 %,7.72 %,3.14 %,11.18 % and 5.22 %.At the same time,the antioxidant activity of the six polysaccharides was determined through the determination of hydroxyl radical scavenging capacity.The results showed that although Agrocybe chaxingu contained the highest of polysaccharide,the weakest of hydroxyl radical scavenging capacity;Flammulina velutiper,Ganoderma lucidum and Lentinus edodes,not only had more polysaccharide,and the ability of clearing hydroxyl radical was better,among them.In comparison,the polysaccharide from Flammulina velutiper has a wider development foreground.