谷氨酸的
海马体
谷氨酸受体
海马结构
突触可塑性
莫里斯水上航行任务
神经传递
水迷宫
内分泌学
受体
神经科学
运输机
巴恩斯迷宫
心理学
内科学
生物
医学
生物化学
基因
空间学习
作者
Mathias Zink,Thomas Ferbert,Stefanie Frank,Patricia Seufert,Peter J. Gebicke‐Haerter,Rainer Spanagel
标识
DOI:10.1111/j.1460-9568.2011.07776.x
摘要
Perinatal exposure to alcohol (PEA) induces general developmental and specific neuropsychiatric disturbances accompanied by disturbed synaptic plasticity. Here we studied the long-term behavioral consequences of PEA and investigated glutamate transmission-related genes in a longitudinal fashion. After delivery, female Wistar rats and their pups were exposed to ethanol until postnatal day (PD)8 in vapor chambers. At the age of 5 months, the animals were behaviorally characterized. At both PD8 and after the behavioral testing we examined the expression of the vesicular glutamate transporter 1 and excitatory amino acid transporter (EAAT)1–4, as well as the N-methyl-d-aspartate receptor subunits (NR)1 and 2A–D, and in parallel receptor binding using 3H-dizocilpine maleate receptor autoradiography. We found highly significant reductions of body weight and length following PEA in pups at PD8. These alterations disappeared in adulthood, when no changes of motor activity and only subtle differences of anxiety-related behavior were observed. It also did not affect T-maze learning, but had a pronounced effect on hippocampus-dependent spatial learning (Morris water maze testing). This specific learning deficit was accompanied by a dysregulation in hippocampal gene expression (significant induction of vesicular glutamate transporter 1, EAAT1, EAAT3, NR2A, 2B, 2C and 2D). Most of the examined genes turned out to be dysregulated to a higher degree at the age of 5 months. We therefore conclude that perinatal ethanol toxicity alters the plasticity of neurodevelopment and the regulation of glutamatergic gene expression, which may result in specific hippocampus-dependent learning deficits in adulthood.
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