Synergistic leaching of heavy metal-polycyclic aromatic hydrocarbon in co-contaminated soil by hydroxamate siderophore: Role of cation-π and chelation

Exploring a novel green efficient bioeluant is a golden key to unlock the ex-situ scale remediation of soil contaminated with heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs). Hydroxamate siderophore (HDS) produced by Pseudomonas fluorescens HMP01, with certain hydrophobicity and strong coordination because of its special chemical structure (e.g., hydroxamic acid and dihydroxy quinoline chromophore), was used to investigate the bioleaching efficiency of HMs and PAHs from actual contaminated soils and underlying mechanisms. Results showed that leaching efficiency for HMs and PAHs from the co-contaminated soil was higher than that of single contaminated soil due to the cation-π interaction and coordination, which was closely related to the spacial configuration changes of the complex. HDS not only increased the bioleaching efficiency of cationic HMs by chelation (the leaching amount of Cd2+, Pb2+, Hg2+, Cu2+, Zn2+, and Ni2+ achieved 27.5, 110.4, 6.9, 477.7, 10,606.9, and 137.4 mg/kg HDS, respectively) but also enhanced the bioleaching amount of PAHs by solubilization (the leaching amount of phenanthrene reached 90.2 mg/kg HDS. Also, the residual HDS in soils caused no significant ecological risk. As expected, HDS is a desirable bioeluant to promote the scale application of the ex-situ remediation of soil contaminated with HMs and PAHs.