草酸盐
草酸钙
甘氨酸
化学
肾结石
体内
生物化学
钙
泌尿系统
柠檬酸
尿
肾
内科学
内分泌学
生物
氨基酸
医学
无机化学
有机化学
生物技术
作者
Lan Yu,Wei Zhu,Xiaolu Duan,Tuo Deng,Shujue Li,Yang Liu,Yang Zhou,Yuan Wen,Lianming Luo,Shankun Zhao,Jiamin Wang,Zhijian Zhao,Wenqi Wu,Guohua Zeng
摘要
An abnormal urine composition is a key reason for kidney stone formation, but little is known about the roles of small metabolites in the urine during kidney stone formation. Here, we found urine glycine in patients with kidney calcium oxalate (CaOx) stone was significantly lower than that in healthy people via 1 H NMR spectra detection, and investigated the role and underlying mechanism of glycine in the regulation of CaOx stone formation. Our results showed that glycine could significantly attenuate ethylene glycol-induced CaOx crystal depositions in rat kidney via decreasing urine oxalate and increasing urine citrate. Mechanism studies revealed that glycine could decrease urine oxalate through downregulating Slc26a6 expression, whereas increase urine citrate via inhibiting Nadc1 expression. Moreover, glycine decreased the protein expression of both Slc26a6 and Nadc1 via increasing the expression of miRNA-411-3p, which directly bound to the 3'-untranslated regions of Slc26a6 and Nadc1 messenger RNAs, in vitro and in vivo. Together, our results revealed a novel role of glycine in the regulation of kidney CaOx crystal formation and provided a potential target for the treatment of kidney CaOx stone.
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