内斯汀
SOX2
生物
人口
神经干细胞
神经球
病理
脊髓
细胞生物学
干细胞
成体干细胞
解剖
神经科学
胚胎干细胞
医学
体外
内皮干细胞
环境卫生
基因
生物化学
作者
Daria Mamaeva,Chantal Ripoll,Claire Bony,Marisa Teigell,Florence E. Perrin,Bernard Rothhut,Ivan Bièche,Rosette Lidereau,Alain Privat,Valérie Rigau,Hélène Guillon,Florence Vachiery-Lahaye,Danièle Noël,Luc Bauchet,Jean-Philippe Hugnot
标识
DOI:10.1186/1471-2202-12-99
摘要
Abstract Background The adult central nervous system (CNS) contains different populations of immature cells that could possibly be used to repair brain and spinal cord lesions. The diversity and the properties of these cells in the human adult CNS remain to be fully explored. We previously isolated Nestin + Sox2 + neural multipotential cells from the adult human spinal cord using the neurosphere method (i.e. non adherent conditions and defined medium). Results Here we report the isolation and long term propagation of another population of Nestin + cells from this tissue using adherent culture conditions and serum. QPCR and immunofluorescence indicated that these cells had mesenchymal features as evidenced by the expression of Snai2 and Twist1 and lack of expression of neural markers such as Sox2, Olig2 or GFAP. Indeed, these cells expressed markers typical of smooth muscle vascular cells such as Calponin, Caldesmone and Acta2 (Smooth muscle actin). These cells could not differentiate into chondrocytes, adipocytes, neuronal and glial cells, however they readily mineralized when placed in osteogenic conditions. Further characterization allowed us to identify the Nkx6.1 transcription factor as a marker for these cells. Nkx6.1 was expressed in vivo by CNS vascular muscular cells located in the parenchyma and the meninges. Conclusion Smooth muscle cells expressing Nestin and Nkx6.1 is the main cell population derived from culturing human spinal cord cells in adherent conditions with serum. Mineralization of these cells in vitro could represent a valuable model for studying calcifications of CNS vessels which are observed in pathological situations or as part of the normal aging. In addition, long term propagation of these cells will allow the study of their interaction with other CNS cells and their implication in scar formation during spinal cord injury.
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