Moment Analysis for Study of Reaction Kinetics between Phenylboronic Acid and Monoccharides by Affinity Capillary Electrophoresis

Abstract A moment analysis method was applied to the study of intermolecular interactions between phenylboronic acid and monosaccharides to demonstrate its effectiveness. The values of first absolute and second central moments of elution peaks measured by affinity capillary electrophoresis (ACE) were analyzed on the basis of the moment theory to determine those of association equilibrium constant and association and dissociation rate constants of the intermolecular interactions. Their values were fairly in agreement with corresponding results reported in previous studies. The moment analysis method using ACE has some advantages in comparison with conventional spectroscopic methods. For example, neither immobilization nor fluorescence labelling of solute and/or ligand molecules is required when ACE data are measured. Because the principle of the method is different from those of ordinary spectroscopic ones, it contributes to the kinetic study of intermolecular interactions from various perspectives. It was also tried to compare some intrinsic characteristics of the moment analysis method with those of two historical ones, which were developed by Whitesides’ group and Krylov’s group. Some restrictive features of the moment analysis method were also explained. It was concluded that the moment analysis method using ACE is effective as one of experimental methods for the analysis of intermolecular interactions.