核梭杆菌
DNA损伤
基底切除修复术
DNA修复
生物
癌症
脆弱类杆菌
结直肠癌
酶
类有机物
癌症研究
下调和上调
微生物学
DNA
基因
细胞生物学
遗传学
牙龈卟啉单胞菌
细菌
生物化学
抗生素
作者
Ibrahim M. Sayed,Anirban Chakraborty,Soumita Das
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 157-172
被引量:1
标识
DOI:10.1007/978-1-0716-3373-1_10
摘要
Microbes play an important role in regulating cellular responses and the induction of chronic diseases. Infection and chronic inflammation can cause DNA damage, and the accumulation of mutations leads to cancer development. The well-known examples of cancer-associated microbes are Helicobacter pylori in gastric cancer and Fusobacterium nucleatum (Fn), Bacteroides fragilis, and E.coli NC101 in colorectal cancer (CRC). These carcinopathogens modify the expressions of the base excision repair enzymes and cause DNA damage. This chapter will show how Fn can initiate CRC through the downregulation of a critical enzyme of the base excision repair (BER) pathway that subsequently causes accumulation of DNA damage. We used the stem cell-based organoid model and enteroid-derived monolayer (EDM) from the murine and human colon to assess the impact of infection on the expression of BER enzymes on the transcriptional and translational levels and to develop other functional assays. For example, we used this EDM model to assess the inflammatory response, DNA damage response, and physiological responses, where we correlated the level of these parameters to BER enzyme levels.
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