Determination of the timing of early gonadal differentiation in silver pomfret, Pampus argenteus

Silver pomfret is a species of global significance due to its high nutritional in fisheries sector. To accurately ascertain the timing of sex differentiation mechanism and mRNA level in this species, this study examined gonad morphology and patterns of gene expression related to sex differentiation in males and females from 0 to 180 days post hatch (dph), the temperature of water was maintained at 26±1℃. Distinct morphological differentiation of the silver pomfret ovaries, marked by the emergence of primary oocytes, became apparent from 68 dph. By 108 dph, the testes began to differentiate, as evidenced by the appearance of the efferent duct. Early oocytes exhibited a diameter ranged from 0.077 mm to 0.682 mm, with an average diameter of 0.343±0.051 mm. The proportions of various types of germ cells within the testes were subjected to analysis. The localization of Vasa during the early stages of sexual differentiation was a subject to analysis as well. Vasa was predominantly localized within the cytoplasm of gonocyte, peri-nucleolus stage oocytes, primary oocytes and type A spermatogonocytes, indicating that Vasa is involved in the early gonadal differentiation of silver pomfret. The study investigated the expression patterns of dmrt1, gsdf, amh, foxl2, cyp19a1a, cyp11a, sox3 and vasa, all of which are involved in the sex differentiation of teleosts. Among these genes, amh, gsdf, sox3, foxl2, vasa were indentified as crucial contributors to the early gonadal development of silver pomfret. Significant sex-related differences were observed in the expression patterns of amh, dmrt1, gsdf, cyp11a, sox3, cyp19a1a, vasa. This study provides novel insights into the timing of physiological changes associated with the sexual differentiation of silver pomfret. Collectively, the Present data indicates that the differentiation of ovaries and testes take place approximately at 68 dph in females and 108 dph in males.