Bioluminescence assay for rapid detection of live Staphylococcus aureus based on the enrichment of egg yolk antibody modified magnetic metal organic framework immunobeads

The main significance and novelty can be summarized: (1) mMOF-IgY efficiently enrich S.A and amplify bioluminescence signal. (2) Lysed S.A emit ATP and produce bioluminescence. (3) The assay can detect S.A in foods with LOD of 3 CFU mL −1 in 30 min.