Green Sample Preparation Techniques and Their Use in the Extraction and Separation Analysis of Phenolic Compounds in Honey

ABSTRACTThis review provides an overview of the current trends and prospects of the extraction and separation analysis techniques for phenolic compounds in honey in 2012–2022 years. The classification, chemical structures, physicochemical, and bioactive properties of phenolic compounds in honey were comprehensively analyzed. The recent sample preparation techniques for extracting and separating the phenolic compounds from honey were discussed. The advantages and disadvantages of different extraction and separation analyses were also analyzed and compared. According to recent literatures, solid phase extraction and liquid-liquid extraction, two traditional sample preparation techniques, are still widely used for extracting phenolic compounds from honey samples. Various improved microscale extraction methods, such as solid phase microextraction and liquid-liquid microextraction, and sub-technologies can be applied considering the recovery rates, costs, solvent consumption, and environmental impacts. This review will provide insights into the extraction and separation analysis of phenolic compounds, and foster the development and utilization of active components in honey.KEYWORDS: Honeyphenolic compound analysisgreen sample preparationextractionseparation AcknowledgmentsThe authors gratefully acknowledge the National Natural Science Foundation of China (Grant No. 32172300),Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThe work was supported by National Natural Science Foundation of China (Grant No. 32172300), the Sponsored by Program for Science & Technology Innovation Talents in Universities of Henan Province (Grant No. 23HASTIT048), and the Hunan Provincial Natural Science Foundation (Grant No. 2021JJ50151).