化学
脱氧核酶
荧光
G-四倍体
分析物
核酸
水溶液
生物传感器
组合化学
选择性
色谱法
DNA
检出限
生物化学
有机化学
催化作用
物理
量子力学
作者
Hui Sun,Liang Zheng,Zhihong Li,Xinyue Ma,Zixuan Duan,Chia‐Chung Sun
标识
DOI:10.1080/00032719.2022.2143793
摘要
A simple and label-free fluorescent sensor based upon a specific DNAzyme (8–17 DNAzyme) and thiazole orange (TO) for monitoring Pb2+ in water samples was described. The 8–17 DNAzyme was formed by an enzyme strand (17E) and a partially complementary substrate strand (17DS). Thiazole orange, the DNA intercalator, was nonfluorescent in aqueous solution while it intercalates into the 8–17 DNAzyme with a duplex structure resulting in strong fluorescence. In the presence of Pb2+, the 17E was activated and cleaved the 17DS into two at the ribonucleoside adenosine (rA) position, leading to the disruption of the duplex structure. This Pb2+-induced conformational change led to the quenching of the fluorescence of thiazole orange, thus forming the basis for the label-free biosensor. Under the optimized conditions, the developed protocol exhibited sensitive determination of Pb2+ with a detection limit of 0.73 nM and a linear range from 10 to 150 nM. The label-free fluorescent approach exhibited excellent selectivity, strong selectivity, and satisfactory analysis of a drinking water standard reference material and spiked river and tap water samples. Taking the advantage of the structure-switching 8–17 DNAzyme and the fluorescent intercalator of nucleic acids, this label-free sensor provides a new approach for routine screening of heavy metals based upon the DNAzyme.
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