结垢
扫描电子显微镜
过滤(数学)
膜
膜污染
无定形固体
环境扫描电子显微镜
化学
滤饼过滤器
化学工程
材料科学
表征(材料科学)
纳米技术
复合材料
色谱法
结晶学
生物化学
统计
数学
工程类
作者
Kevork Oliver Messerian,Anton Zverev,Jack F. Kramarczyk,Andrew L. Zydney
标识
DOI:10.1016/j.memsci.2023.121896
摘要
The recent success of mRNA vaccines has generated increased interest in the use of Lipid Nanoparticles (LNPs) for delivery of nucleic acids. The objective of this study was to characterize the deposit formed on the surface of membranes during sterile filtration of mRNA-LNPs using a combination of scanning electron microscopy (SEM), environmental scanning electron microscopy (ESEM), atomic force microscopy (AFM), and hydraulic resistance measurements. The microscopic characterizations showed an amorphous deposit fouling the surface of the filter and blocking the membrane pores. The resistance of the deposit showed an unusual biphasic behavior, increasing at low pressure (consistent with a compressible filtration medium) but decreasing at high pressure, in a reversible manner. The surprising reduction in resistance at high pressure has not previously been identified in the filtration literature. The pressure corresponding to the maximum resistance (Pcritical) was found to be proportional to the inverse of the membrane pore size, as described by the Young-Laplace relationship. The hydraulic resistance measurements and microscopic characterization are consistent with a mechanism whereby an amorphous fouling deposit intrudes through membrane pores at high transmembrane pressures. These results provide important insights into the physical properties of the fouling deposit that governs the sterile filtration of LNPs.
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