Optimizing Biosynthesis of Nicotinamide Mononucleotide, a Potential Nutritional Supplement, by E. coli

Nicotinamide mononucleotide plays a crucial role in the biosynthesis of the coenzyme nicotinamide adenine dinucleotide, which is involved in various metabolic pathways in all living organisms. Nicotinamide mononucleotide has recently received attention for its potential health benefits in aging, weight loss, diabetes, and as a neuroprotectant. In this study, we aimed to optimize the production of nicotinamide mononucleotide in E. coli with combinations of genes and plasmids. Our results show that nicotinamide mononucleotide production in Escherichia coli is highly related to bacterial growth rate, indicating that the highest nicotinamide mononucleotide production can be achieved by strains with moderate growth rates. This finding highlights the importance of maintaining a balanced growth rate to achieve optimal nicotinamide mononucleotide production in E. coli. Our study provides insights into optimizing the in vivo synthesis of nicotinamide mononucleotide, a potential agent for nutritional supplementation for novel therapies in various age-related diseases.