脱落酸
恶毒的
分解代谢
突变体
黄瓜
MADS箱
细胞生物学
腋芽
抑制因子
调节器
生物
拟南芥
植物
生物化学
转录因子
新陈代谢
基因
体外
外植体培养
作者
Jiacai Chen,Liu Liu,Guanghui Wang,Guangxin Chen,Xiaofeng Liu,Min Li,Lijie Han,Weiyuan Song,Shaoyun Wang,C K Li,Zhongyi Wang,Yuxiang Huang,Chaoheng Gu,Zhengan Yang,Zhaoyang Zhou,Jianyu Zhao,Xiaolan Zhang
标识
DOI:10.1093/plcell/koae108
摘要
Abstract Lateral branches are important components of shoot architecture and directly affect crop yield and production cost. Although sporadic studies have implicated abscisic acid (ABA) biosynthesis in axillary bud outgrowth, the function of ABA catabolism and its upstream regulators in shoot branching remain elusive. Here, we showed that the MADS-box transcription factor AGAMOUS-LIKE 16 (CsAGL16) is a positive regulator of axillary bud outgrowth in cucumber (Cucumis sativus). Functional disruption of CsAGL16 led to reduced bud outgrowth, whereas overexpression of CsAGL16 resulted in enhanced branching. CsAGL16 directly binds to the promoter of the ABA 8'-hydroxylase gene CsCYP707A4 and promotes its expression. Loss of CsCYP707A4 function inhibited axillary bud outgrowth and increased ABA levels. Elevated expression of CsCYP707A4 or treatment with an ABA biosynthesis inhibitor largely rescued the Csagl16 mutant phenotype. Moreover, cucumber General Regulatory Factor 1 (CsGRF1) interacts with CsAGL16 and antagonizes CsAGL16-mediated CsCYP707A4 activation. Disruption of CsGRF1 resulted in elongated branches and decreased ABA levels in the axillary buds. The Csagl16 Csgrf1 double mutant exhibited a branching phenotype resembling that of the Csagl16 single mutant. Therefore, our data suggest that the CsAGL16–CsGRF1 module regulates axillary bud outgrowth via CsCYP707A4-mediated ABA catabolism in cucumber. Our findings provide a strategy to manipulate ABA levels in axillary buds during crop breeding to produce desirable branching phenotypes.
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