Ингибиторный анализ активности NADPH-оксидазы в хемоаттрактант-индуцированной дегрануляции нейтрофилов человека

The objective of the present study was to characterize the influence of diphenylene iodonium and apocynin, “oxidative burst” inhibitors, on degranulation of neutrophils obtained from the healthy donors. The oxidative burst was measured by the luminal-dependent chemiluminescence technique. Diphenylene iodonium and apocynin, a selective inhibitor of NADPH-oxidase, practically completely down-regulated the oxidative burst induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP) within the range of concentration used in the study. The same reactants caused a dose-dependent reduction of exocytosis of the azurophilic and specific granules stimulated by a FMLP agonist. The results of the study are discussed in the context of the electrogenic activity of the NADPH-oxidase enzymatic complex capable of depolarizing the neutrophil plasma membrane and thereby affecting various proinflammatory functions including exocytosis of the granules.