果胶
多糖
化学
萃取(化学)
糖
汤剂
食品科学
产量(工程)
大小排阻色谱法
色谱法
植物
生物
有机化学
材料科学
冶金
酶
作者
Mohamad Khatib,Camilla Giuliani,Federico Rossi,Alessandra Adessi,Amal Al-Tamimi,Giuseppe Mazzola,Diana Di Gioia,Marzia Innocenti,Nádia Mulinacci
标识
DOI:10.1016/j.foodchem.2017.05.041
摘要
The main crude polysaccharides (CPS), extracted from two widely cultivated pomegranate varieties, Laffan and Wonderful, were studied and characterized. We obtained the highest CPS extraction yield (approximatively 10% w/w on dried matter) by 1h of decoction (ratio 1/40w/v). The predominant polymers (75-80%) of the CPS samples showed a hydrodynamic volume close to 2000kDa by size exclusion chromatography and the exocarp and mesocarp profiles were very similar. The proton spectra (1H NMR), according to sugar composition and gelling ability, confirmed the main polysaccharide fractions were pectin with different acylation and methylation degree. The CPS from Laffan and Wonderful mesocarp showed prebiotic properties in vitro with Lactobacillus and Bifidobacterium strains. The composition of the decoction (12% ellagitannins and 10% of CPS) obtained by a green extraction process of pomegranate by-products, makes it a suitable component of functional food formulations.
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