单核苷酸多态性
纳米团簇
化学
DNA
SNP公司
荧光
核苷酸
二聚体
点突变
DNA测序
碱基对
基因
计算生物学
遗传学
突变
生物
基因型
物理
生物化学
有机化学
量子力学
作者
Jie Liu,Yuexiang Lu,Feng Lu,Song Wang,Shixi Zhang,Xuewei Zhu,Linfeng Sheng,Sichun Zhang,Xinrong Zhang
标识
DOI:10.1021/acs.analchem.6b04981
摘要
Single nucleotide polymorphisms (SNPs) are the most fundamental internal causes for many genetic diseases. However, the location information on SNPs in a specific DNA sequence is not well acquired through current SNPs detection methods, except for accurate DNA sequencing. Here we report a fluorescence enhancement phenomenon in the process of two silver nanoclusters (AgNCs) approaching closely to form a nanocluster dimer (NCD). The fluorescence intensity is sensitive to the distance between two AgNCs; therefore, the NCD lights into different fluorescence intensities upon binding SNPs targets with mismatched bases at different positions. Interestingly, the fluorescence intensities of the NCD decrease linearly when the position of single mismatched base moves gradually from the middle point to the end of the target DNA. The NCD is a single probe acting as a universal platform to pinpoint various SNP positions. With this single probe, we cannot only identify the existence of SNPs but also pinpoint the location of a specific single mismatched base in the adjacent positions. This strategy is feasible to detect specific gene point mutations in clinical samples.
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