Cellular fluorescence imaging based on resonance energy transfer

Fluorescence imaging of intracellular active molecules plays an important role in understanding the occurrence and development of diseases. Resonance energy transfer (RET) has been recognized as a powerful technology in the design of fluorescence probes for biological imaging. Here, we summarize the novel approaches to fabrication of RET-based fluorescent probes systems such as the organic molecular fluorescent probe, nano fluorescent probe, protein probe and their recent applications in cellular fluorescence imaging.