光动力疗法
过氧化氢酶
过氧化氢
活性氧
材料科学
细菌
体内
缺氧(环境)
癌症研究
氧气
生物物理学
纳米技术
黑磷
肿瘤缺氧
医学
化学
生物
细胞生物学
放射治疗
生物化学
酶
生物技术
光电子学
有机化学
内科学
遗传学
作者
Shuaijie Ding,Zeming Liu,Chunyu Huang,Ning Zeng,Wei Jiang,Qing Li
标识
DOI:10.1021/acsami.0c20254
摘要
Intratumoral hypoxia significantly constrains the susceptibility of solid tumors to oxygen-dependent photodynamic therapy (PDT), and effort to reverse such hypoxia has achieved limited success to date. Herein, we developed a novel engineered bacterial system capable of targeting hypoxic tumor tissues and efficiently mediating the photodynamic treatment of these tumors. For this system, we genetically engineered Escherichia coli to express catalase, after which we explored an electrostatic adsorption approach to link black phosphorus quantum dots (BPQDs) to the surface of these bacteria, thereby generating an engineered E. coli/BPQDs (EB) system. Following intravenous injection, EB was able to target hypoxic tumor tissues. Subsequent 660 nm laser irradiation drove EB to generate reactive oxygen species (ROS) and destroy the membranes of these bacteria, leading to the release of catalase that subsequently degrades hydrogen peroxide to yield oxygen. Increased oxygen levels alleviate intratumoral hypoxia, thereby enhancing BPQD-mediated photodynamic therapy. This system was able to efficiently kill tumor cells in vivo, exhibiting good therapeutic efficacy. In summary, this study is the first to report the utilization of engineered bacteria to facilitate PDT, and our results highlight new avenues for BPQD-mediated cancer treatment.
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