生化工程
污染
生物负载
计算机科学
风险分析(工程)
纳米技术
生物技术
医学
工程类
生物
材料科学
外科
生态学
作者
Pierre Prada,Benjamin Brunel,Fany Reffuveille,Sophie C. Gangloff
出处
期刊:Applied sciences
[Multidisciplinary Digital Publishing Institute]
日期:2022-06-09
卷期号:12 (12): 5892-5892
被引量:13
摘要
Rapid detection of microorganisms is a major challenge in the medical and industrial sectors. In a pharmaceutical laboratory, contamination of medical products may lead to severe health risks for patients, such as sepsis. In the specific case of advanced therapy medicinal products, contamination must be detected as early as possible to avoid late production stop and unnecessary costs. Unfortunately, the conventional methods used to detect microorganisms are based on time-consuming and labor-intensive approaches. Therefore, it is important to find new tools to detect microorganisms in a shorter time frame. This review sums up the current methods and represents the evolution in techniques for microorganism detection. First, there is a focus on promising ligands, such as aptamers and antimicrobial peptides, cheaper to produce and with a broader spectrum of detection. Then, we describe methods achieving low limits of detection, thanks to Raman spectroscopy or precise handling of samples through microfluids devices. The last part is dedicated to techniques in real-time, such as surface plasmon resonance, preventing the risk of contamination. Detection of pathogens in complex biological fluids remains a scientific challenge, and this review points toward important areas for future research.
科研通智能强力驱动
Strongly Powered by AbleSci AI