应力颗粒
蛋白激酶R
冠状病毒科
维罗细胞
病毒复制
病毒学
生物
病毒
冠状病毒
核糖核酸
免疫系统
磷酸化
蛋白激酶A
免疫学
细胞生物学
医学
翻译(生物学)
2019年冠状病毒病(COVID-19)
信使核糖核酸
生物化学
丝裂原活化蛋白激酶激酶
疾病
病理
基因
传染病(医学专业)
作者
Haipeng Feng,Jingyan Zhang,Kang Zhang,Xuezhi Wang,Kai Zhang,Zhuang Guo,Songwei Han,Lei Wang,Zhengying Qiu,Guibo Wang,Jianxi Li
标识
DOI:10.1016/j.intimp.2022.108764
摘要
The prevalence of avian infectious bronchitis virus (IBV) is still one of causes inducing severe losses of production in the poultry industry worldwide. Vaccination does not completely prevent IBV infection and spread due to immune failure and viral mutations. Forsythiae Fructus and its compounds have been widely used in a lot of prescriptions of the traditional Chinese medicine for a long history, and it is well-known as safety and efficiency in heat-clearing and detoxifying. This study aims to investigate the anti-IBV activity and mechanism of phillygenin. The results showed that phillygenin inhibited IBV replication by disturbing multiple stages of the virus life cycle, including viral adsorption, invasion, internalization, and release in Vero cells. After being treated with 100, 125 and 150 μg/mL phillygenin, the expression of G3BP1 was significantly increased and the phosphorylation of PKR/eIF2α was activated, which increased stress granule, thereby triggering the antiviral response in Vero cells. The anti-virus activity of PHI was decreased when G3BP1 was interfered by si-RNA, and G3BP1 was down-regulated when PKR/eIF2α was interfered by si-RNA. In conclusion, our findings indicate that phillygenin activates PKR/eIF2α pathway and induces stress granule formation to exert anti-IBV, which holds promise to develop into a novel anti-IBV drug. Further study in vivo is needed to explore phillygenin as a potential and effective drug to prevent IB in poultry.
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