亚硫酸盐
喹啉
荧光
化学
水溶液
光化学
溶酶体
质谱法
费斯特共振能量转移
分析化学(期刊)
无机化学
色谱法
生物化学
物理化学
有机化学
酶
物理
量子力学
作者
Xiaohong Li,Xuefeng Han,Wei-Na Wu,Xiao-Lei Zhao,Yuan Wang,Yun-Chang Fan,Zheng Xu
标识
DOI:10.1016/j.saa.2022.121160
摘要
A lysosome-targeting ratiometric fluorescent probe was synthesized for detecting sulfite based on sulfite-triggered nucleophilic addition reaction. Due to the specific reaction, the fluorescence intensity ratio (I530/I390) of the probe in an almost aqueous solution (0.5% DMSO) changed significantly after the addition of HSO3-, corresponding to the change in the fluorescence color of the solution from green to blue. The recognition was conducted using high-resolution mass spectrometry, proton nuclear magnetic resonance, and density functional theory calculations. The fluorescent probe could be utilized to quantitatively monitor HSO3- in lysosomes of living C6 glioma cells and real-water samples.
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