脱氧核酶
检出限
肉眼
辣根过氧化物酶
DNA
分子信标
线性范围
滚动圆复制
纳米技术
核酸
胎儿游离DNA
化学
材料科学
生物化学
寡核苷酸
色谱法
生物
酶
DNA聚合酶
胎儿
遗传学
产前诊断
怀孕
作者
Shaowei Liu,Yumeng Yang,Mengqin Shi,Hai Shi,Dali Mao,Xiaoxia Mao,Yuanguang Zhang
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-02-02
卷期号:7 (2): 658-665
被引量:18
标识
DOI:10.1021/acssensors.1c02662
摘要
Cell-free DNA (cfDNA), as a tumor marker, is of great importance for the diagnosis of cancer and targeted therapy. However, the need for huge analytical instruments for cfDNA analysis has restricted its practical applications, especially in rural areas and third-world countries. Herein, a portable and visual smartphone-based DNAzyme hydrogel platform is developed for cfDNA detection. The target cfDNA triggers rolling circle amplification to produce a G-quadruplex-comprised DNA hydrogel with an horseradish peroxidase (HRP)-like catalytic function, which further catalyzes the chromogenic substrate to generate a visible output signal. Notably, the naked-eye detection of cfDNA can be realized by the macroscale visibility and catalytic ability of the DNA hydrogel. The linear range of the DNAzyme hydrogel platform for cfDNA detection is 0.1 pM-1500 nM with a detection limit of 0.042 pM. Moreover, this platform is exploited for the detection of cfDNA in spiked human serum with favorable sensitivity and recovery. Therefore, the DNAzyme hydrogel platform provides highly promising potential for testing other nucleic acid biomarkers.
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