光遗传学
生物物理学
斑马鱼
肌醇
视紫红质
化学
细胞生物学
生物
生物化学
神经科学
视网膜
基因
受体
作者
William Benman,Erin E. Berlew,Hao Deng,Caitlyn Parker,И. А. Кузнецов,Bomyi Lim,Arndt F. Siekmann,Brian Y. Chow,Lukasz J. Bugaj
标识
DOI:10.1038/s41589-021-00917-0
摘要
We describe single-component optogenetic probes whose activation dynamics depend on both light and temperature. We used the BcLOV4 photoreceptor to stimulate Ras and phosphatidyl inositol-3-kinase signaling in mammalian cells, allowing activation over a large dynamic range with low basal levels. Surprisingly, we found that BcLOV4 membrane translocation dynamics could be tuned by both light and temperature such that membrane localization spontaneously decayed at elevated temperatures despite constant illumination. Quantitative modeling predicted BcLOV4 activation dynamics across a range of light and temperature inputs and thus provides an experimental roadmap for BcLOV4-based probes. BcLOV4 drove strong and stable signal activation in both zebrafish and fly cells, and thermal inactivation provided a means to multiplex distinct blue-light sensitive tools in individual mammalian cells. BcLOV4 is thus a versatile photosensor with unique light and temperature sensitivity that enables straightforward generation of broadly applicable optogenetic tools.
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