软骨发生
组织工程
软骨
自愈水凝胶
生物医学工程
软骨细胞
脚手架
细胞生物学
再生(生物学)
化学
间充质干细胞
细胞外基质
透明软骨
再生医学
生物材料
材料科学
阿格里坎
干细胞
糖胺聚糖
去细胞化
基质(化学分析)
透明质酸
骨关节炎
作者
Bo Huang,Pinxue Li,Mingxue Chen,Peng Liqing,Luo Xujiang,Guangzhao Tian,Hao Wang,Liping Wu,Tian Qinyu,Hao Li,Yu Yang,Jiang Shuangpeng,Zhen Yang,Kangkang Zha,Xiang Sui,Liu Shuyun,Quanyi Guo
标识
DOI:10.1186/s12951-021-01230-7
摘要
Abstract Background The regeneration and repair of articular cartilage remains a major challenge for clinicians and scientists due to the poor intrinsic healing of this tissue. Since cartilage injuries are often clinically irregular, tissue-engineered scaffolds that can be easily molded to fill cartilage defects of any shape that fit tightly into the host cartilage are needed. Method In this study, bone marrow mesenchymal stem cell (BMSC) affinity peptide sequence PFSSTKT (PFS)-modified chondrocyte extracellular matrix (ECM) particles combined with GelMA hydrogel were constructed. Results In vitro experiments showed that the pore size and porosity of the solid-supported composite scaffolds were appropriate and that the scaffolds provided a three-dimensional microenvironment supporting cell adhesion, proliferation and chondrogenic differentiation. In vitro experiments also showed that GelMA/ECM-PFS could regulate the migration of rabbit BMSCs. Two weeks after implantation in vivo, the GelMA/ECM-PFS functional scaffold system promoted the recruitment of endogenous mesenchymal stem cells from the defect site. GelMA/ECM-PFS achieved successful hyaline cartilage repair in rabbits in vivo, while the control treatment mostly resulted in fibrous tissue repair. Conclusion This combination of endogenous cell recruitment and chondrogenesis is an ideal strategy for repairing irregular cartilage defects. Graphical Abstract
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