In the satellite navigation and positioning system,Doppler frequency shift is the key problems that degrade the performance of satellite receiver.To overcome the influence of Doppler frequency shift and improve the speed of GPS signal acquisition,the paper first studies the algorithm of Doppler frequency shift estimation,and then deduces the mathematic models of satellite movement paremeters and Doppler frequency shift estimation.The paper has analyzed visible satellite's determination,the Doppler frequency deviation computational method,principle test platform based on the NewStar 150GPS,using the C++ language programming,and has developed the Doppler frequency deviation computational procedure.The experimental result indicated that the Doppler frequency deviation's size and the angle concern.When α90°,Doppler shift for positive,the user receiver receives the frequency must be lower than the satellite launch's frequency.When α90°,the Doppler shift was negative;when α=90°,the Doppler shift was zero.