[Study on optimizing the extraction process of total free organic acids in Pinellia ternate Breit].

OBJECTIVE: To optimize the extraction process of total free organic acids in Pinellia ternate Breit. METHOD: The optimum extraction was investigated by total free organic acids determined by direct potential titration. The concentration of ethanol, amount of ethanol, extraction time and extraction times were the four factors in the experiment. RESULTS: The optimum extraction process was adding 10 times amount of 75% ethanol, refluxing for 2 times, 1 h each time. CONCLUSION: This extraction process shows higher yield of total free organic acids in Pinellia ternate Breit and is available for industrial production.