生物
保幼激素
蜜蜂
表观遗传学
DNA甲基化
卵黄原蛋白
遗传学
基因
甲基转移酶
甲基化
基因表达调控
基因表达
内分泌学
动物
激素
作者
Carlos Antônio Mendes Cardoso-Júnior,Karina Rosa Guidugli-Lazzarini,Klaus Hartfelder
标识
DOI:10.1016/j.ibmb.2017.11.005
摘要
The canonic regulatory module for lifespan of honey bee (Apis mellifera) workers involves a mutual repressor relationship between juvenile hormone (JH) and vitellogenin (Vg). Compared to vertebrates, however, little is known about a possible role of epigenetic factors. The full genomic repertoire of DNA methyltransferases (DNMTs) makes the honey bee an attractive emergent model for studying the role of epigenetics in the aging process of invertebrates, and especially so in social insects. We first quantified the transcript levels of the four DNMTs encoding genes in the head thorax and abdomens of workers of different age, showing that dnmt1a and dnmt3 expression is up-regulated in abdomens of old workers, whereas dnmt1b and dnmt2 are down-regulated in heads of old workers. Pharmacological genome demethylation by RG108 treatment caused an increase in worker lifespan. Next, we showed that the genomic DNA methylation status indirectly affects vitellogenin gene expression both in vitro and in vivo in young workers, and that this occurs independent of caloric restriction or JH levels, suggesting that a non-canonical circuitry may be acting in parallel with the JH/Vg module to regulate the adult life cycle of honey bee workers. Our data provide evidence that epigenetic factors play a role in regulatory networks associated with complex life history traits of a social insect.
科研通智能强力驱动
Strongly Powered by AbleSci AI