同源盒蛋白纳米
重编程
SOX2
细胞生物学
胚状体
KLF4公司
细胞效价
细胞分化
作者
Shingo Miyawaki,Yohei Okada,Hideyuki Okano,Kyoko Miura
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2017-08-20
卷期号:7 (16)
被引量:2
标识
DOI:10.21769/bioprotoc.2518
摘要
Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze et al., 2009 ; Gropp et al., 2012 ). On the other hand, teratoma-forming tumorigenicity also represents a major risk factor impeding potential clinical applications of pluripotent stem cells ( Miura et al., 2009 ; Okano et al., 2013 ). Recently, we reported that iPSCs derived from naked mole-rat lack teratoma-forming tumorigenicity when engrafted into the testes of non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice due to an ES cell-expressed Ras (ERAS) and Alternative reading frame (ARF)-dependent tumor-suppression mechanism specific to this species ( Miyawaki et al., 2016 ). Here, we describe a method for transplanting pluripotent stem cells into the testes of NOD/SCID mice to generate teratomas for assessing the pluripotency and tumorigenicity.
科研通智能强力驱动
Strongly Powered by AbleSci AI