萝卜
大豆黄酮
植物毒性
阿卡波糖
染料木素
化学
阿维链霉菌
链霉菌
芥子酶
IC50型
立体化学
体外
生物化学
酶
发酵
植物
芸苔属
生物
硫代葡萄糖苷
细菌
内分泌学
遗传学
作者
Jing Wei,Xiuyun Zhang,Shan Deng,Lin Cao,Qiao Xue,Jin‐Ming Gao
标识
DOI:10.1080/14786419.2016.1269100
摘要
Twenty-four metabolites 1–24 were isolated from the fermentation broth of Streptomyces xanthophaeus. Their structures were elucidated on the basis of spectroscopic analysis and by comparison of their NMR data with literature data reported. Daidzein (1), genistein (2) and gliricidin (3) inhibited α-glucosidase in vitro with IC50 values of 174.2, 36.1 and 47.4 μM, respectively, more potent than the positive control, acarbose. Docking study revealed that the amino acid residue Thr 215 is the essential binding site for active ligands 2. In addition, the phytotoxic effects of all compounds were assayed on radish seedlings, five of which, 3, 8, 13, 15 and 18, inhibited the growth of radish (Raphanus sativus) seedlings with inhibitory rates of >60% at a concentration of 100 ppm, which was comparable or superior to the positive control glyphosate. This is the first report of the phytotoxicity of the compounds.
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