精液
微泡
纳米粒子跟踪分析
外体
CD63
化学
精子
萃取(化学)
超离心机
色谱法
分子生物学
生物
细胞生物学
生物化学
基因
遗传学
小RNA
作者
Cheng Yang,Wenbin Guo,Wansong Zhang,Jun Bian,Jing Yang,Tao Qi,Chunyan Wang,Cundong Liu
出处
期刊:PubMed
日期:2016-11-20
卷期号:36 (11): 1531-1535
被引量:3
摘要
To extract and identify semen-derived exosome using PEG6000.Exosomes were extracted from semen specimens from 6 healthy volunteers with step-by-step centrifugations and ultracentrifugation prior to 8% PEG6000 enrichment. The extracted exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting.The pellets obtained were round or elliptic membrane vesicles 30 to 150 nm in diameter with intact double membranes and contained low electron density material. The pellets expressed CD63, ALIX and TSG101 molecules but not calnexin that was expressed in sperm cells.The PEG6000-based method for extraction of exosomes from semen samples facilitates future studies of seminal exosomes.
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