光热治疗
化学
大肠杆菌
光热效应
生物传感器
检出限
适体
致病菌
滚动圆复制
纳米技术
细菌
DNA
色谱法
分子生物学
DNA聚合酶
生物化学
材料科学
基因
生物
遗传学
作者
Jing Jing Zhang,Chao Nie,Wen Long Fu,Feng Li Cheng,Pu Chen,Zhong Feng Gao,Yongning Wu,Yizhong Shen
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-11-17
卷期号:94 (48): 16796-16802
被引量:14
标识
DOI:10.1021/acs.analchem.2c03714
摘要
The development of facile, reliable, and accurate assays for pathogenic bacteria is critical to environmental pollution surveillance, traceability analysis, prevention, and control. Here, we proposed a rolling circle amplification (RCA) strategy-driven visual photothermal smartphone-based biosensor for achieving highly sensitive monitoring of Escherichia coli (E. coli) in environmental media. In this design, E. coli could specifically bind with its recognition aptamer for initiating the RCA process on a magnetic bead (MB). Owing to the cleaving of UV irradiation toward photoresponsive DNA on MB, the RCA products were released to further hybridize with near-infrared excited CuxS-modified DNA probes. As a result, the photothermal signal was enhanced by RCA, while the background was decreased by UV irradiation and magnetic separation. The correspondingly generated photothermal signals were unambiguously recorded on a smartphone, allowing for an E. coli assay with a low detection limit of 1.8 CFU/mL among the broad linear range from 5.0 to 5.0 × 105 CFU/mL. Significantly, this proposed biosensor has been successfully applied to monitor the fouling levels of E. coli in spring water samples with acceptable results. This study holds great prospects by integrating a RCA-driven photothermal amplification strategy into a smartphone to develop accurate, reliable, and efficient analytical platforms against pathogenic bacteria pollutions for safeguarding environmental health.
科研通智能强力驱动
Strongly Powered by AbleSci AI