超声
萃取(化学)
三螺旋
傅里叶变换红外光谱
胶原螺旋
溶解度
圆二色性
化学
Ⅰ型胶原
扫描电子显微镜
变性(裂变材料)
核化学
结晶学
材料科学
色谱法
化学工程
立体化学
有机化学
复合材料
医学
工程类
病理
作者
Özge Ata,Bahar Bakar,Burcu Kaplan Türköz,Seher Kumcuoğlu,Y. Aydoǧdu,Barış Gümüştaş,Gizem Dinler Doğanay,Ezgi Basturk,Şebnem Tavman
标识
DOI:10.1111/1750-3841.16870
摘要
Abstract This study aimed to extract collagen‐I from lamb feet (LF) and examine the effects of ultrasound treatment on the structural and molecular characteristics of the collagen. Compared to ultrasonic bath treatment and conventional extraction methods, ultrasonic probe (USP) treatment significantly increased the collagen content of the extract ( p < 0.05). The electrophoretic profiles confirmed the presence of α‐ and β‐chains, indicating it as type I. Furthermore, X‐ray diffraction, Fourier‐transform infrared spectroscopy, and circular dichroism spectra analyses revealed that the extraction method did not adversely affect the triple helix structure of the collagen. Moreover, the fibrillar structure of the collagen samples was verified through scanning electron microscopy analyses. Notably, the LF collagen exhibited a high thermal denaturation temperature owing to its elevated imino acid content. The collagen samples exhibited high solubility in acidic pH but low solubility in high salt concentrations. The present findings signified that sonication with USP can effectively enhance the yield of collagen from LF without compromising its quality. Practical Application This study showed that ultrasonication enhanced the collagen concentration without disturbing the integrity of lamb feet collagen. We expect that lamb feet collagen can be used for industrial processes and consumer products thanks to unique product properties.
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