Impact of fermented feed of soybean hulls and rapeseed cake on immunity, antioxidant capacity, and gut microbiota in Chahua chicken

The present study investigated the effects of replacing part of the basal diet with two-stage fermented feed (FF) (soybean hulls : rapeseed cake (2 : 1, m/m)) on the growth performance, immunity, antioxidant capacity, and intestinal health of Chahua chicken . A total of 160 Chahua chickens were randomly divided into four groups to receive control diet or diet with 5%, 10%, or 15% of the basal diet replaced by FF, respectively for 56 days. The results showed that FF significantly improved the average daily gain (ADG) and average daily feed intake (ADFI) of Chahua chickens (P < 0.05). Furthermore, the serum immunoglobulin (Ig) A, glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in Chahua chicken receiving the diet added with 15% FF significantly increased (P < 0.05). Chahua chicken in both 10% and 15% group showed increased serum IgG and IgM and decreased malondialdehyde. Serum interleukin-2 and interferon-gamma significantly increased in all FF groups. Compared with the CON group, higher ileal villus height (VH) was found in the 10% FF group . Treatment with FF significantly increased the ileal villus height/crypt depth (VH/CD) ratio, jejunal VH, and jejunal VH/CD ratio while reducing ileal and jejunal CD. The modified gut microbiota composition was observed in Chahua chicken fed diet containing with FF, in particular, with the increased abundance of Faecalibacterium and Lactobacillus. The abundance of Lactobacillus significantly increased in the 10% and 15% FF groups (all P < 0.05). Correlation analysis revealed a positive correlation between Lactobacillus and VH (R = 0.38, P = 0.10, Figure 3B), AH/CD ratio (R = 0.63, P = 0.003), and a negative correlation with CD (R = -0.72, P = 0.001). These above results indicate that FF improves immunity, antioxidant capacity, and intestinal health and consequently enhance growth performance in Chahua chicken.