Integrated LC‐MS and Network Pharmacology Reveal Metabolic Profile and Quantitative Analysis of Active Components in Coptidis Rhizoma–Aucklandiae Radix Herb Pair Between Healthy and Diarrheal Mice

A total of 45 components were identified in the extract of CR-AR. Among them, 16 prototype compounds and 47 metabolites were identified, and potential metabolic pathways (including hydroxylation, demethylation, reduction, hydrolysis, hydrogenation, and glucuronidation) were proposed. Based on the 16 prototype components, six potentially active components (berberine, jatrorrhizine, palmatine, columbamine, epiberberine, and dehydrocostus lactone) were screened from the prototype components-targets-pathways-AAD disease network. Targeted quantitative analysis showed that alkaloid-based active components were significantly more concentrated in the intestines of AAD mice than in healthy mice after 6 h (p < 0.05).