Targeted DNA N6‐methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

Graphical Abstract We have established a novel CRISPR–dCas9–METTL4 epigenome editing tool that can methylate target regions to achieve site-specific DNA 6mA methylation in both hypermethylated and hypomethylated genes. Targeted methylation on genes by dCas9–METTL4 results in misexpression, allowing for the functional investigation of target genes of interest in silkworm.