信使核糖核酸
生物
转录组
突变体
转录因子
基因
细胞生物学
抄写(语言学)
核糖核酸
基因表达
分子生物学
生物化学
语言学
哲学
作者
Alexandra G. Zonnefeld,Chang‐Yi Cui,Dimitrios Tsitsipatis,Yulan Piao,Jinshui Fan,Krystyna Mazan-Mamczarz,Yutong Xue,Fred E. Indig,Supriyo De,Myriam Gorospe
出处
期刊:Aging
[Impact Journals, LLC]
日期:2024-04-17
标识
DOI:10.18632/aging.205776
摘要
Evaporation of sweat on the skin surface is the major mechanism for dissipating heat in humans. The secretory capacity of sweat glands (SWGs) declines during aging, leading to heat intolerance in the elderly, but the mechanisms responsible for this decline are poorly understood. We investigated the molecular changes accompanying SWG aging in mice, where sweat tests confirmed a significant reduction of active SWGs in old mice relative to young mice. We first identified SWG-enriched mRNAs by comparing the skin transcriptome of Eda mutant Tabby male mice, which lack SWGs, with that of wild-type control mice by RNA-sequencing analysis. This comparison revealed 171 mRNAs enriched in SWGs, including 47 mRNAs encoding 'core secretory' proteins such as transcription factors, ion channels, ion transporters, and trans-synaptic signaling proteins. Among these, 28 SWG-enriched mRNAs showed significantly altered abundance in the aged male footpad skin, and 11 of them, including Foxa1, Best2, Chrm3, and Foxc1 mRNAs, were found in the 'core secretory' category. Consistent with the changes in mRNA expression levels, immunohistology revealed that higher numbers of secretory cells from old SWGs express the transcription factor FOXC1, the protein product of Foxc1 mRNA. In sum, our study identified mRNAs enriched in SWGs, including those that encode core secretory proteins, and altered abundance of these mRNAs and proteins with aging in mouse SWGs.
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