类有机物
神经科学
人脑
免疫染色
皮质(解剖学)
视皮层
生物
感觉系统
免疫学
免疫组织化学
作者
Madison Wilson,Martin Thunemann,Xin Liu,Yichen Lu,Francesca Puppo,Jason W Adams,Jeong-Hoon Kim,Mehrdad Ramezani,Donald Pizzo,Srdjan Djurovic,Ole A. Andreassen,Abed AlFatah Mansour,Fred H. Gage,Alysson R. Muotri,Anna Devor,Duygu Kuzum
标识
DOI:10.1038/s41467-022-35536-3
摘要
Abstract Human cortical organoids, three-dimensional neuronal cultures, are emerging as powerful tools to study brain development and dysfunction. However, whether organoids can functionally connect to a sensory network in vivo has yet to be demonstrated. Here, we combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid. Visual stimuli evoke electrophysiological responses in the organoid, matching the responses from the surrounding cortex. Increases in multi-unit activity (MUA) and gamma power and phase locking of stimulus-evoked MUA with slow oscillations indicate functional integration between the organoid and the host brain. Immunostaining confirms the presence of human-mouse synapses. Implantation of transparent microelectrodes with organoids serves as a versatile in vivo platform for comprehensive evaluation of the development, maturation, and functional integration of human neuronal networks within the mouse brain.
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