Green Synthesis of Silver Nanoparticles Using Plant (Azadirachtaindica) Leaf Extract

Silver nanoparticles were green synthesized from freshly prepared Azadirachtaindica (Neem) leaves broth at room temperature. An aqueous solution of silver nitrate (AgNO 3 ) was treated with neem leaf extract. The silver nanoparticles formed were characterised using UV-Visible spectrophotometer and Transmission Electron Microscopy. The UV-Visible absorption peak at 427 nm clearly indicates the formation of Silver nanoparticles. The TEM images confirmed that the silver nanoparticles were spherical in shape and their size ranged between 50-200 nm. Silver nanoparticles were exhibited effective anti-microbial activity against Escherichia coli. Toxicity of silver nanoparticles were carried out using MTT (3-(4, 5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide) dye assay indicates considerable cytotoxic effect against chicken bone marrow cells.