Effects of exogenous L-Glutamine as a sole nitrogen source on physiological characteristics and nitrogen use efficiency of poplar

L-Glutamine (Gln) is a proteinogenic amino acid, N transporter and NH3 carrier, engaging in diversified pathways for synthesizing many important molecules. However, the effects of exogenous Gln on plant growth and development remain largely unknown. In this study, different concentrations of Gln were supplemented in the poplar hybrid 'Nanlin895' culture medium as a sole N source. Their effects on poplar growth, photosynthesis, N metabolism-related enzymes and metabolites were elucidated. Strikingly, 0.5 mM Gln-fed poplars showed no considerable growth compromise compared to the inorganic N control (CK-N), even though their N supply level was only half that of the CK-N control. What's more, their NUE was enhanced. In addition, 0.5 mM Gln treatment significantly increased the contents of amino acids in coordination with soluble sugars in the roots, while marginal effects in the leaves were observed compared to CK-N. By contrast, applying a high level of Gln (>0.5 mM) resulted in larger accumulation of amino acids and starch, but lower level of soluble sugars, particularly in the roots, followed by adverse effects on poplar biomass, photosynthesis, enzyme activities and NUE; consequently, poplar growth was inhibited. Collectively, these findings allow us to deduce that poplar plants are competent to take up and utilize Gln as a sole N source. When applied at an appropriate level, Gln could promote a dynamic equilibrium of N and C, conferring sound growth performance and additional benefit for the environment as indicated by higher NUE, lower N input and higher biocompatible nature than the inorganic N.