HPLC simultaneous determination of two effective components in “Xiakucao Oral Solution”

Objective To establish the HPLC method for simultaneous determination of the caffeic acid and rosmarinic acid inXiakucao Oral Solution. Methods Detecting condition: C18column( 4. 6 mm × 250 mm,5 μm) and the gradient of MeOH-0. 1% acetic acid solution was taken as the mobile phase at a flow rate of 1. 0 ml /min,the detection wavelength was 330 nm and column temperature at 30 ℃. Results The linear ranges of caffeic acid and rosmarinic acid were 0. 056~ 0. 728μg( r = 1. 0) and 1. 65 ~ 21. 45μg( r = 1. 0) respectively. The average recovery rate of caffeic acid and rosmarinic acid were 101. 2% and 98. 8%respectively( RSDs were 1. 33% and 1. 31% respectively,n = 6). Conclusion The experimental results showed that the method could be used to determine the effective components including caffeic acid and rosmarinic acid inXiakucao Oral Solution.