The inheritance laws and physiological variation of resistance to verticillium wilt in a new cotton germplasm Lu HB22 were studied by inoculation with V991 Verticillium dahliae strain.The results indicated that the resistance to verticillium wilt at seedling stage was controlled by a single dominant gene.Compared to the susceptive control Jimian 11,the rapid increase and longer keeping time of high PAL activity after inoculation may be one of the main physiological mechanisms resistant to verticillium wilt in Lu HB22.The variation of MDA content after inoculation with Verticillium dahliae,especially lower than that of susceptive control Jimian 11 from the third day after inoculation,could be regarded as one of the biochemical indices to distinguish the resistance to verticillium wilt of Lu HB22.