Study on extraction of bromelain from pineapple

The method of extracting bromelain by traditional method and new method were compared.The proteins content in the enzyme was calculated by Folin-hydroxybenzene measure,18mg/g and 21.5mg/g.The enzymes activation was calculated with the casein as the substrate,3.29×10~6U/g and 3.33×10~6U/g.The optimum pH and temperature for activating bromelain are 7.4 and 45℃.The effects of various compounds on the enzyme activity were showed that EDTA had important effect on protecting the bromelain.