Determination of Naringin,Hesperidin and Baicalin in Tongxuan Lifei Capsules

Objective:To establish an HPLC method for determination of naringin,hesperidin and baicalin in Tongxuan Lifei capsules. Method:The chromatographic column of Agilent ZORBAX SB C18( 4. 6 mm × 250 mm,5 μm) was used,the mobile phase was consisted of acetonitrile-0. 1% phosphoric acid aqueous solution( 21:79) .The flow rate was 1. 0 mL· min -1,the detective wavelength was 280 nm and column temperature was 30 ℃ .Result: The linear range of naringin,hesperidin and Baicalin were 0. 156-1. 408 μg( r=0. 999 8) ,0. 145-1. 301 μg( r=0. 999 7) and 0. 359-3. 233 μg( r=0. 999 9) ,the average recoveries (n=9) were 99. 56% ( RSD 0. 88% ) ,were 99. 83% ( RSD 0. 66% ) and 98. 94% ( RSD 1. 55% ) respectively. Conclusion: The results showed thismethod is accurate,reproducible,simple and easy to do,which can be used in quality control of Tongxuan Lifei Capsules.