The effect of phosphorus source, e. g. dipotassium hydrogen phosphate and disodium beta-glycerophosphate, on the growth of Microcystis aeruginosa and the microcystin generation was studied. The cell number, total phosphorus contents in medium and in the cell of Microcystis aeruginosa, pH of the medium, microcystin -LR and microcystin -RR content were analyzed during the growth of Microcystis aeruginosa. Results showed that the algae cell number and microcystin levels using disodium beta-glycerophosphate as phosphorus source were lower than that of dipotassium hydrogen phosphate which indicated that dipotassium hydrogen phosphate could promote the growth of Microcystis aeruginosa, and was favorable to the generation of intracellular microcystin.