Upregulation of extraneuronal TRPV1 expression in chronic rhinosinusitis with nasal polyps

Upregulation of extraneuronal TRPV1 expression in chronic rhinosinusitis with nasal polyps* Background: Chronic rhinosinusitis (CRS) is a multifactorial upper airway disease with unclear etiology.Neuronal Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) channels have been implicated in the pathogenesis of CRS.We aimed to detect the expression of extraneuronal TRPV1 and TRPA1 receptors in nasal polyp (NP) tissue samples.Methodology: Samples were obtained from fourty-two CRS pateints with nasal polyp and sixteen healthy controls to measure receptor gene expression by quantitative PCR, protein localization by immunohistochemistry and cytokine profile by multiplex bead immunoassay.Results: Non-neuronal TRPV1, TRPA1 receptors were expressed in biopsy samples of NP.A population of mast cells and macrophages were immunopositive for TRPV1 and TRPA1.A fraction of plasma cells expressed TRPV1 but not TRPA1 and neither receptor was present on eosinophils.The local gene expression of extraneuronal TRPV1, TRPA1 receptors was also proven.TRPV1 mRNA levels were significantly increased in CRSwNP patients with asthma and allergic rhinitis compared to their NP counterparts.Conclusions: Elevated TRPV1 levels in comorbid asthma and allergy may have a function in CRSwNP.Subpopulation-specific TRPV1 presence on plasma and mast cells can indicate delicate roles in regulating activation and release of inflammatory mediators.