Antifouling and conducting PEDOT derivative grafted with polyglycerol for highly sensitive electrochemical protein detection in complex biological media

化学 生物污染 佩多:嘘 缩水甘油 电化学 导电聚合物 生物传感器 聚合 单体 蛋白质吸附 表面改性 组合化学 高分子化学 有机化学 聚合物 电极 生物化学 物理化学 催化作用
作者
Linzheng Ma,Silambarasan Jayachandran,Zimeng Li,Zhen Song,Wei Wang,Xiliang Luo
出处
期刊:Journal of Electroanalytical Chemistry [Elsevier BV]
卷期号:840: 272-278 被引量:29
标识
DOI:10.1016/j.jelechem.2019.04.002
摘要

Abstract A novel biomaterial, polyglycerol grafted conducting polymer poly (3,4-ethylenedixythiophene) (PEDOT) was electrochemically synthesized. The PEDOT functionalized with hyperbranched polyglycerol (PEDOT-HPG) was simply prepared through the electrochemical polymerization of corresponding monomers, which was synthesized by grafting glycidol into (2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methanol (EDOT-MeOH). The antifouling properties of PEDOT-HPG surfaces were evaluated in terms of protein adsorption and the attachment of mammalian cells, including human cervical carcinoma cells (HeLa) and Michigan Cancer Foundation-7 (MCF-7). Through the conjugation of alpha-fetoprotein (AFP) antibodies to the PEDOT-HPG surfaces, sensitive immunosensors for AFP were fabricated, with the limit of detection at the level of 0.035 pg/mL. The prepared AFP biosensors exhibited excellent antifouling performance even in complex media and showed promising feasibility for the quantitative analysis of AFP in real samples. The PEDOT derivative with hyperbranched polyglycerol groups was effective to reduce biofouling and facile for surface functionalization. It is expected that this novel antifouling and conducting material may find broad applications in the development of electrochemical sensors and biosensors capable of assaying targets in complex biological media.

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